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Importantly, recent studies aimed at blocking the metaorganismal TMAO pathway through multiple approaches have confirmed the inhibition of diet-dependent atherosclerosis in animal models, either by targeting the initial microbial formation of TMA from choline through use of a nonlethal small-molecule inhibitor (19), or by targeting the second step in the pathway, the major host gene responsible for converting microbe-generated TMA into TMAO, flavin monooxygenase 3 (20, 21)

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All absorption/fluorescence measurements were performed using a 96-well plate reader (Synergy H1 Hybrid Reader, BioTek, Winooski, VT, USA) at specified wavelengths (see below)

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