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Description
For long-term maintenance (after day 35), the spheres were transferred to ultralow attachment plates (Corning, VWR) at five spheres per ml of media

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Brain Stimulation

Both uniformly C13-labeled palmitic acid (UC13-C16:0, Larodan) and docosahexaenoic acid (DHA, Cayman Chemicals) were conjugated with albumin (using Fatty Acid Free Bovine Serum Albumin, BSA, Goldbio) to a final concentration of 2 mM and 40 M, respectively, and aliquoted out into 5 ml glass scintillation vials stored at 20C
