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While the cells treated with Ru(II)-OH or NP2 in a 1% O 2 atmosphere were confluent and alive, the cell treated with NP4 had a strongly reduced cell population and a mixture of living and dead cells comparable to the treatment with these nanoparticles in a 21% O 2 atmosphere (Fig

For establishing human hepatocyte cultures, fresh or cryopreserved human hepatocytes, prepared from human liver fragments, were obtained from Biopredic International (Saint-Grgoire, France)
