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In animal-model work, NNMT inhibition with 5-Amino-1MQ has been associated with changes in adipose-tissue mass, in NAD+ pool sizes (because NNMT consumes nicotinamide that would otherwise feed the NAD+ salvage pathway), and in methyl-donor dynamics (because NNMT consumes S-adenosylmethionine)

Generally, NNMT overexpression decreases SAM and S-adenosyl-L-homocysteine (SAH) levels and alters SAM/SAH in vivo and in vitro in an obvious manner (96)

Recommendation: Treatment frequency determined during consultation

The trial adequately powered to detect a 3-4 kg difference with the dosing regimen used (PMID: 23714218)