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or, when R 1 is C 1 -C 6 alkyl or C 1 -C 6 alkyl substituted by one or more than one R 1a , the C 1 -C 6 alkyl is independently methyl
Briefly, HEK 293 T cells (Thermo Scientificl #HCL4517) were transfected with the calcium phosphate method with an equimolar mixture of the expression plasmid, pHelper plasmid, and Rep/Cap plasmid pAAV2/DJ (for the production of the shRNA, pAAV2/1 and pAAV2/2 were combined to obtain a mixed capsid of AAV1/2)
doi:10.1016/j.ctim.2018.09.003
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