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2.6.5 Capillary-based Western blot To validate protein level changes, capillary-based Simple Western assays were performed in RePlex mode using the Jess device (Protein Simple, San Jose, CA, United States) according to the manufacturers protocol, employing an immunoassay followed by a total protein assay for normalization

In responders, cholesterol pathway connected the signals sent from SELENOP-macrophages to the other cell clusters in the network, while interferon pathway connected signals sent from CD4 Helper T cells to the other cell clusters

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