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The mass spectrometer was operated in data-dependent mode with the following acquisition cycle: an MS scan (m/z 3501600) recorded at resolution R = 60,000 and MS/MS scans recorded at resolution R = 15,000, which were acquired by HCD (higher-energy C-trap dissociation fragmentation) with collision energy of 28.MS/MS spectra were searched with the Mascot engine (v2.6, Matrix Science) against the UniProtKB mouse protein database using the following parameters: a precursor peptide mass tolerance of 20 ppm and an MS/MS fragment tolerance of 0.02 Da with a maximum of two missed cleavage sites

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