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Callway CW (2013): Epinephrine for cardiac arrest

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the antibody (R1401-12) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system

However, the results show that the accuracy of this unsupervised learning method is much lower than traditional supervised learning methods

Myers, but it remains the most popular IV protocol
