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One hypothesis is that we observed a greater induction of NRF2 through liver-specific Gclc deletion as compared to KEAP1 inhibition with CDDO-me because CDDO-me is a weaker inhibitor of NRF2-KEAP1 interaction compared to the ROS generated following GSH depletion

Irisin activates SIRT1 to deacetylate HIF-1, reducing lactate metabolism and cell apoptosis To explore how irisin regulates HIF-1 through SIRT1, we used co-immunoprecipitation to isolate HIF-1 and measured its acetylation status

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Selenium SeLECT selenium (L-selenomethionine)