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The basic principle of this method is to analyze the respiratory capacity at 37 C in a hyper oxygenated environment with multiple substrate uncouplers

mitochondrial membrane potential (e.g., via JC-1 staining) reflects shifts in cellular energy state

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It is important to remember that a healthy microbiome is associated with high microbiota diversity and a balanced bacterial composition ( 2.9.1 Clinical studies The hypothesis that gut diseases influence the CNS is supported by the finding that colon biopsies from patients with PD display higher CD3 + T cell infiltration, upregulated expression of the proinflammatory cytokines interleukin-1 (IL-1) and interferon-gamma, and elevated calreticulin levels in mucosal tissue and feces