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CRISPRCas9 method to generate KO cell lines To construct PDIA6 -KO HCT116 cells, using px330-PuroR 64 as a template, the DNA oligonucleotides 5-CGGTGTTTCGTCCTTTCCACAAGATATATA-3 and 5-AAGGACGAAACACCGACTTCTCGGTTGAAATTCGAGTTTTAGAGCTAGAAATAGCAAG-3 or 5- AAGGACGAAACACCGAACACCATACTGACCTCCTAGTTTTAGAGCTAGAAATAGCAAG-3 were utilized for inverse PCR to express guide RNAs targeting exons 2 and 4, respectively, of the PDIA6 gene

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doi: 10.1016/j.biopha.2020.110663

22 GSH is essential for innate and adaptive immune functions The functions of GSH are not only inhibitory as described above for the inflammatory response