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The sample then underwent 3 cycles of freeze-thawing followed by extrusion (Avestin Liposofast LF-50) once at 65C through a 200 nm membrane (Cytiva Nuclepore) then 3X through 100 nm membranes (Cytiva Nuclepore)
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P401, P1138 Huayi H
Alpha Lipoic Acid: This is a water-and fat-soluble organic compound and antioxidant, which is synthesized in the mitochondria
