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Shimatzu 10AVP HPLC System (Shimatzu, Kyoto, Japan) with manual sample injector was used to run the HPLC-UV method on an isocratic gradient with mobile phase comprising of 10 mM 1-heptane sulfonate, 20 mM potassium phosphate monobasic [pH 3.1], 4% methanol, and 3% acetonitrile
