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Isolation and Structure Determination of a Candidate Tetradepsipeptide Ethyl Ester ( m/z 453) and Octadepsipeptide Ethyl Ester ( m/z 837), Respectively The combined EtOH extracts obtained from cultures (100 mL) of strain F4810/72 were concentrated to about 25 mL in vacuum and, after dilution with H 2 O (1/10, v/v), aliquots (5 mL) were separated by means of preparative RP-HPLC on a PrepStar system (Varian, Darmstadt, Germany) consisting of two HPLC-pumps (Model SD-1), a two-wavelength UV detector (Prostar 325), a fraction collector (Model 701) and equipped with a 250 21.2 mm, 4 m, 90A, Jupiter Proteo column (Phenomenex) as the stationary phase

Concentration (mcg/mL) = Total Peptide (mcg) divided by Water Volume (mL) Example: You have a 5 mg vial of BPC-157 (that is 5,000 mcg)

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Compared to the rest of the EL3VATE research-peptide range, BPC-157 occupies the soft-tissue-repair lane (alongside TB-500), separate from the metabolic lane (retatrutide) and the mitochondrial lane (SS-31)