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Investigation of glutathione reductase and 25-hydroxylase for clearing aggregated proteins in an experimental fish eye cataract model Fiona Tsai (1), Huiru M Huang (1), Chang Sun Lee (1), Jason Hsu (1), Megan Yang (1), Avery Wang (1), Leon Yim (1), Alvin E Wang* (1), Chih Heng Lai (1), Angela Y Lu (1), Jacqueline Chein (1), Hellen Jang (1), Austin Lai (1), Apollo Lo (1), Justin Chang (1), Moksha Shah (1), Kevin Liao (1), Justin J Yang (1), Catherine Yeh (1), Yvonne Wei (1), Jubi Y Lin (1), Andrew Chen (1), Dennis Jang (1), Zach Verne (1), Stephanie Cheng (1), Sean Tsao (1), Teresa Chiang (1), Jude C Clapper (1) Taipei American School, Taipei City, Taiwan

Wilshire CE, Kindlon T, Courtney R, Matthees A, Tuller D, Geraghty K, et al

YS: Data curation, Formal Analysis, Writing original draft

After 3 h, the supernatant was incubated with 30 l of protein G agarose Fastflow for an extra 1 h, washed extensively with lysis buffer containing 250 mM NaCl (spun at 3000 rpm for 1 min)