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Low quality score bases and adaptor sequences were first trimmed using Trim Galore (version 0.2.7)

(PubMed) Yin G, Ming H, Zheng X, Xuan Y, Liang J, Jin X

Briefly, gallic acid of known concentration was used as a standard and Folin-Ciocalteu reagent was allowed to react with different concentrations of standard and sample for 5 min, Na2CO3 was then added, mixed thoroughly, and incubated at room temperature in the dark for 2 h
A Double-Blind, Randomized, Placebo-Controlled Study