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doi:10.1007/s00431-005-0033-8

Vector-bearing Caco-2 cells were selected via resistance to hygromycin B after cDNAs for CYP450 were introduced into an extrachromosomal vector under the control of the cytomegalovirus early intermediate promoter to develop Caco-2 cell expressing high levels of CYP450 enzymes ( 3 (1,25-(OH) 2 -D 3 ), beginning at the confluence, results in a dose- and duration-dependent increase in CYP3A4 mRNA and protein ( via these technologies

Kuzminski AM, Del Giacco EJ, et al

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