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Assessing protein expression by SDS-PAGE and western blotting Cellular fractions (mitochondria, cytosol, 3050 g protein) from the liver and kidneys of control Wistar and diabetic GK rats were electrophoretically separated by 7.5%12% SDS-PAGE (Laemmli, 1970) and transferred onto nitrocellulose membrane (Towbin et al., 1992)

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