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10.3390/nu15234852 Nutrients 103 PalmerJ

2.11 In situ detection of mitochondrial and superoxide levels Fresh bladder tissue from each group was prepared into frozen sections and incubated briefly at 37 C in the dark with Mito-Tracker Red, 2,7-dichlorodihydrofluorescein diacetate (DHE, 10 M, Beyotime) and MitoSOX Red (10 M, Invitrogen) for 30 min at 37 C in the dark
Stevens KN, Creanor S, Jeffery A, Whone A, Zajicek J, Foggo A, et al