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For example, macrophages produce an array of arachidonic acid-derived prostaglandins in response to Toll-like receptor (TLR) stimuli but the specific prostaglandins and their quantities produced after stimulation differ dramatically between macrophages of different tissue origin and isolation method for example, thioglycollate-elicited peritoneal macrophages and bone marrow-derived macrophages, as well as between primary macrophages and a transformed macrophage cell line 23