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10.3892/mmr.2020.11631 38 GuoX.BaiX.LiL.LiJ.WangH
Its dysregulation promotes uncontrolled proliferation, stemness, metastasis and therapy resistance
For intracellular cytokine, staining cells were fixed with BD Cytofix Cytoperm (BD Biosciences) for 20 min at 4 C, washed twice with 1x saponin buffer (0.5% (w/v) saponin [Carl Roth], 2 mM EDTA, 2% FCS, in PBS) and stained for IFN (see Table 1) overnight at 4 C in 1 saponin buffer
J.BallardC.Ray ChaudhuriK.et al (2021)
