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Personal or family history of auto-immune disease for instance, thyroid disease, vitiligo, type 1 diabetes, Sjogrens disease (dry mouth and other symptoms), Addisons disease

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The A-side buffer contained 100 M Lucifer yellow dye, in transport buffer (1.98 g/L glucose in 10 mM HEPES, 1 Hanks balanced salt solution, pH 7.4), and the B-side buffer was transport buffer at pH 7.4