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Furthermore, asymmetric flow field-flow fractionation is now being used to isolate and characterize HDL (765)

Meanwhile, a culture of EcN pSIM18 was grown to mid-log phase at 30 C in LB + 200 g/mL hygromycin, then expression of the lambda Red proteins was induced by incubating the cells at 42 C for 15 min, followed by 15 min on ice

Both 25-hydroxylases (CYP2R1 and CYP27A1) were expressed in the liver, while CYP27A1 expression was seen only in cultured hepatocytes (FL83B mouse hepatocyte cell line)

Moreover, they influence immune recruitment and migration by altering the synthesis of chemokines and adhesion molecules (271, 276)