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After 24 h postplating time, fresh media were supplied (200 l/well) and treated with either an equal volume of sterile water (vehicle control) or with 23.4 mmol/l of ICA-1s

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This schedule is convention, not evidence an animal mcg/kg value cannot be converted directly into a human dose without formal allometric and clinical work [3]

(See Figure L) Figure L Step 13: Push the plunger all the way up (See Figure M)
