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Next, we detected the GPX4 and FTH1 levels in colorectal cancer patients undergoing neoadjuvant chemoradiotherapy by immunohistochemistry, and found both protein levels were up-regulated, compared with the patients without neoadjuvant chemoradiotherapy ( Figure 6A )
For cells treated with ML226, a final concentration of 2.5 M was maintained throughout the assay to prevent washout
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