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Protein was eluted with 10 mM glutathione in 50 mM Tris, pH 8.0, 5% glycerol, and examined for purity by SDS-PAGE and Coomassie staining

Studies have found that in Nrf2 knockout rats, the protective effect of CDDO-Im on AFB1 hepatotoxicity is significantly weakened, which indicates that Nrf2 act a pivotal part in this protection (Taguchi et al., 2016)

But it remains to be proved whether patients or the elderly are more likely to have adverse events than the general