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Imaging was performed on an EVOS Digital Inverted Fluorescence Microscope (Invitrogen), using the FITC (green) and the DAPI (blue) filters

What to do with leftover medication from the old concentration When you switch concentrations mid-vial, you may have leftover medication in the old vial

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These results suggest that, when comprehensive multimodal analgesia is used, neither femoral nor sciatic nerve block confers superior analgesic benefit for this procedure, supporting block selection based on motor-sparing goals, technical considerations, or clinician preference rather than on expected analgesic superiority