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Purified sample was crosslinked with 1 mM bis(sulfosuccinimidyl)suberate (BS3) (Thermo) for 45 min at room temperature and quenched with 20 mM Tris-HCl pH 8

Iron overload enhances LPO through Fenton chemistry, whereby free iron catalyzes hydrogen peroxide conversion into highly reactive hydroxyl radicals, promoting oxidative damage to cellular membranes

These antibodies are commonly detected through immunoassays for anticardiolipin (aCL) and anti-2-glycoprotein-I (a2GPI) antibodies, or lupus anticoagulant tests

doi: 10.1126/sciadv.abh0050 27 ZhangJLiZLuHShiJGaoRMaYet al
