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Strong AP, Haeusler S, Weatherall M, Krebs J (2016) Sublingual vitamin B12 compared to intramuscular injection in patients with type 2 diabetes treated with metformin: a randomized trial

Given the vast number of atoms in a protein and the low natural abundance of the most suitable isotopes (e.g., 13 C, 15 N), protein-observed NMR spectroscopy typically involves the production and purification of a uniform isotope-labeled protein along with recording a reference spectrum of the unbound protein to enable the assignment of peaks to specific protein atoms [160,161,162]

Do not apply a heat source, place it in the microwave, or warm it with hot water

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