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Proteins in liver tissues or cell lysates were electrophoretically separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a nitrocellulose membrane, and blocked with skimmed milk powder and incubated at 4 C overnight with primary antibodies

Eggers test p -value was greater than 0.05 for all considered indicators, indicating that studies were unbiased despite high levels of heterogeneity

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Furthermore, hepatitis B virus (HBV) synergizes with AFB1 to exacerbate DNA double-strand breaks and increase TP53 R249S mutation frequency