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Activation of IGF-1 and insulin signaling pathways ameliorate mitochondrial function and energy metabolism in Huntingtons Disease human lymphoblasts

LC-MS/MS analysis was performed by the Metabolomics Facility at Vienna BioCenter Core Facilities (VBCF), which is a member of the Vienna BioCenter (VBC) and funded by the City of Vienna through the Vienna Business Agency

Inmunoprecipitation of crosslinked-Protein/DNA complexes was achieved by using protein G magnetic beads (ThermoFischer), which were incubated at 4 C for 3 h in a rotation mixer with anti-NRF2 as antibody of interest and normal mouse IgG as negative control (see Supplementary Table 7 for antibodies)

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