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A functional R domain from cystic fibrosis transmembrane conductance regulator is predominantly unstructured in solution
SOS activates Ras, a small guanosine triphosphatase (GTPase), by exchanging guanosine diphosphate (GDP) for guanosine triphosphate (GTP)
As we did not observe a clear increase in fluorescence intensity at 405 nm excitation our results indicated that a decrease in glutathione levels was responsible for the lower roGFP2 fluorescence intensity
