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The indicator cells were cultivated overnight with methionine (in the sulfur auxotroph) and GSH (in the gsh1 strain) in the pre-cultures and starved without these sources for 5-6 hours before re-inoculating at an initial OD 600 nm of 0.15 in media containing 100 M of GSH (for the sulfur auxotrophic strain) and no GSH for the glutathione deficient strain

However, because B12 bound to haptocorrin is not metabolically active, use of only total serum B12 may result in Black patients being incorrectly identified as having a high B12 status

It's usually mild and passes quickly

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