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To achieve adipocyte differentiation, 3T3-L1 pre-adipocytes were incubated with differentiation medium (DMEM (1X) + GlutaMAX with 10% fetal bovine serum (FBS) and 1 10 5 U/L penicillin and streptomycin, containing insulin 10 6 M, dexamethasone 10 7 M and 3-isobutyl-1-methylxanthine 10 3 M to starting adipogenesis (day 0)
The Wake Forest University School of Medicine Alzheimers Disease Research Center is coordinating the study, which is sponsored by the Alzheimers Drug Discovery Foundation (ADDF)
The patient will lie on the treatment bed to enhance the calming effects of the room
