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The cells were washed with PBS twice before staining with X-gal solution [1 mg/mL 5-bromo-4-chloro-3inolyl--D-galactoside (Boehringer Manneheim #745-740) in N,N-dimethylformamide, 5 mM potassium ferricyanide crystalline (#P-8131), 5 mM potassium ferricyanide trihydrate (#P-3289), 2 mM magnesium chloride, and 1x PBS, pH 6.0

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Bacteriostatic water for injection: Formulation, uses, and safety considerations

Moreover, digital endpoints (e.g., gait variability, voice modulation, tremor amplitude) using AI analytics offer objective and continuous monitoring over traditional scales like the Unified Parkinsons Disease Rating Scale (UPDRS)