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Description
Gut microbes are the source of various neuroactive substances and neurotransmitters, including short-chain fatty acids, GABA, norepinephrine, dopamine, histamine, serotonin, and acetylcholine (23)

Second step is to ensure the observation by carrying out the laboratory tests

Also are they the right ones

Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components