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In the next reaction, 2-methyl-3-hydroxybutyryl-CoA is metabolized to 2-methylacetoacetyl-CoA by hydroxysteroid 17-beta dehydrogenase 10 which is encoded by the HSD17B10 gene

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A 10-fold increase of plasma TMAO was noted after 12 weeks of supplementation and remained elevated for the next 12 weeks [10]

Fish, Diets and Sampling In order to avoid the metabolic disturbance of estrogen during the sexual maturation of female fish, only male zebrafish (0.25 0.03 g) were bought from Chinese National Zebrafish Resource Center (Wuhan, China)