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As the key enzyme metabolizing APAP into NAPQI, cytochrome P450 enzymes are also considered to be intriguing target for the treatment of APAP-induced liver injury

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For the protein carbonyl immunoassay, inner ear tissues were homogenized using a tissue grinder (Wheaton Dounce Tissue Grinder, Fisher Scientific) containing 1 ml of Tris buffer (10 mM Tris, 1 mM EDTA, 320 mM sucrose, pH 7.4) on ice and then centrifuged at 15,000 g for 10 min at 4 C