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Sample Preparation Animals were fasted overnight and for clinical chemistry blood samples from each rat were collected from the eyes by retro-orbital puncture using blood capillary tubes without heparin as per requirement under mild ether anesthesia blood samples were incubated at room temperature for 10 minutes and left to clot then centrifuged at 3000 r.p.m for 10 min and the serum were collected, serum was separated and kept in clean stopper plastic vial at 80C until the analysis of serum parameters

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10.1016/j.ijcard.2013.12.218 [DOI] [PubMed] [Google Scholar] 23.Berger, A., & Alford, K