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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI

In addition, GHK-treated irradiated fibroblasts showed higher production of growth factors, which are essential for wound healing [31]

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Myoclonus refers to rapid, sudden, shock-like involuntary jerking movements affecting small muscles or the entire body (Tai and Truong, 2015) and tonic-clonic seizures refers to muscle stiffening (tonic phase), followed by rhythmic jerking movements (clonic phase) ( TABLE 1 Modified Racines scale