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Detection wavelengths were set at 220 nm for ITC-Cys and ITC-Cyclic-Cys conjugates, 280 nm for ITC-CysGly conjugates, and 240 nm for ITC-Lys conjugate

Although the exact mechanism of ALS is unknown, recent studies underlined the significance of autophagy in misfolded proteins' clearance and suggested that impaired autophagy may be the basis for ALS onset [113]

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The chromatograph system was equipped with a trap column (Acclaim PepMapTM 100, 75 m 2 cm, C18, 3 m, 100 ) and an analytical column (Acclaim PepMapTM RSLC, 75 m 25 cm, C18, 2 m, 100 )
