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it was then subcultured to OD 600 0.05 in air-saturated medium and shaken vigorously under oxic conditions for 40 min

[DOI] [PMC free article] [PubMed] [Google Scholar] 548.Suomalainen, A

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We've created some detailed video tutorials on our associated YouTube channel that walk through this exact reconstitution process, showing the proper technique for each step