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TNF- was measured using an ELISA kit from R&D Systems (Minneapolis, MN)
Dissolve Gently Swirl 30-60 seconds
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Enzyme-linked immunosorbent assay (ELISA) For comparing antigenicity, purified StrepII-tagged E2 (1.0 g/mL in TBS) or E2E1-I53-50A (equimolar E2, i.e., 2.1 g/mL in TBS) were coated for 2 h at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences)
