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Remove any air bubbles by gently tapping the syringe and pushing them back into the vial before withdrawing your needle
The cells were voltage-clamped at 80 mV, and the ligand was applied using an ultrafast perfusion system comprised of a two-barrel theta pipette mounted on a piezoelectric device
However, such approaches are less likely to be useful clinically in of themselves due to lack of precisely-defined outcome measures and considerable overlap between disease groups
