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Briefly, MCS activity was measured at 30 C using the reaction buffer: 50 mM Tris, pH 7.4, 150 mM NaCl 2 , 2 mM 5,5-dithio-bis-(2-nitrobenzoic acid (DTNB), 0.5 mM OAA, 0.3 mM propionyl-CoA

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