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Annexin V-FITC (2.5 L) and 7-AAD (2.5 L, 100 g/mL) reagents were added, and, following a gentle vortex, the cells were incubated for 20 min
However, their effectiveness is highly dependent to patient breathing pattern and requires patient coordination between actuation of the device and the breath pattern
Luminescence was measured using a BioTek Synergy HT microplate reader
W., Doyle, P
