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Description
Enzymes such as [FeFe] hydrogenases and aminoacyl-tRNA synthetases are amenable to CFE-based screening and engineering using these approaches
The two-electron reducing agent is NADH and the enzyme requires a FAD cofactor close to the active-site Hg-binding cysteines, which operate through a mechanism possibly similar to that of the enzyme glutathione reductase ( vide infra ) [92,93,94]
Yu L, Zheng S, Chen Y, et al
Annu Rev Pharmacol Toxicology
