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Although it was observed for the first time in 1922, in the experiments on fluorescence behavior of a vapor composed of mercury and thallium atoms conducted by Gnther Cario (18971984) and James Franck (18821964) (Cario and Franck, 1922) and the finalized theory was introduced in 1940s by Theodor Frster (19101974) (Forster, 1946) following the earlier work by Francis Perrin (Perrin, 1932), FRET became a go-to technique in the 1960s, when it was demonstrated that FRET measurements could be carried out with fluorescently labeled peptides, and thereby the ruler for molecular interactions was discovered (Stryer and Haugland, 1967

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The goal is always a concentration where your typical dose falls between 5 and 50 units on the syringe, the zone where measurement accuracy is highest

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