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Description
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The results were not subjective assessments but machine-measured dermal changes

Significantly, CuO NP-treated cultures demonstrated enhanced secondary metabolite synthesis, with maximum total phenolic compounds (156.4770.167 mg/g DW GAE) and flavonoids (58.3070.179 mg/g QE) at 8 and 10 mg/L CuO NPs, respectively

Total Time: 45-60 minutes Frequency: Typically 1-2 sessions weekly initially, then maintenance every 4-6 weeks
