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Description
Another practical catch: EGF is a fragile protein so it reacts badly to heat and the wrong pH

Testis were homogenized in 50 mM NaCl, 1 mM EDTA, 1% Triton-X-, and 20-mM TRIS-HCl pH 7.4, by using a IKA Ultra-Turrax homogenizer
This was followed by Yuri Aoyamas 1996 landmark work, which characterized the full-length amino acid sequences of human and rat sterol 14-demethylase and revealed a high degree of homology with the fungal CYP51 enzyme

Not for medical, household or any other uses, for lab use only, Please test before use.
